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eman1_programs

EMAN1 Individual Programs

There are over 200 command-line programs in EMAN, this list contains more detailed documentation for the most commonly used programs. See the fixed documentation for a full list.

GUI Programs

eman Image browser and tutorial interface
boxer Manual particle picking
ctfit CTF parameter determination and simulation
v4 Viewing 3-D models in projection
qsegment Interactive slice-by-slice 3D model segmentation
qindex 2D crystal preprocessing and evaluation
helixboxer Program for picking and evaluating non-square 'boxes'

Refinement Programs

refine2d.py Make high-quality 2-D class-averages from stacks of raw particle images
refine 3-D model refinement
eotest Even/odd data split for resolution evaluation after refinement
multirefine Simultaneous multiple model 3-D refinement

Utility Programs

proc2d Generic 2-D image processing
proc3d Generic 3-D image processing
procpdb.py Simple PDB file manipulation
iminfo Dump information about image files
speedtest Simple application-specific computer benchmarking program
lstcat.py Generate pseudoimage text files
lstfast.py Optimize pseudoimage files for rapid access
pdbtomrc Convert PDB files to MRC density maps
align2d Align 2-D particles to a common reference
align3d Aligns a 3-D model to a similar 3-D model
align3dsym Align a 3-D model so symmetry axes are along canonical axes
volume Compute or adjust the volume of a 3-D reconstruction

Refinement-related Processing

batchboxer Command-line automatic particle picking and box manipulation
fitctf Automatic CTF parameter determination
applyctf Perform phase flipping and other CTF-correction operations
sfmerge.py Merge a low resolution 1-D structure factor from ctfit with a high resolution solution scattering curve
makeinitialmodel.py Interactive script for generating initial models out of Gaussian blobs
startcsym Generate initial models from data with Cn and Dn symmetries
starticos Generate initial models from data with icosahedral (532) symmetry
startoct Generate an initial model from data with octahedral (432) symmetry
startAny Initial model generation using common-lines. Usually a bad idea…
ptcltrace Observe particle orientation changes during refinement
segment3d Automatic segmentation of 3-D reconstructions

AIRS Tools (Analysis of Intermediate Resolution Structures)

foldhunter.py Dock a PDB file or density segment in a 3-D reconstruction
ssehunter.py Identify/locate secondary structural elements in subnanometer resolution reconstructions
helixhunter.py Program to locate alpha-helices in subnanometer resolution reconstructions
skeleton Skeletonization of 3-D models based on both lines and planes
eman1_programs.txt · Last modified: by steveludtke