NCMI Workshop on Single Particle Reconstruction, Validation and Analysis

March 14 - 17, 2011

This page contains the workshop schedule, and will contain the PDFs of each of the presentations at the workshop. Speakers are asked to upload their presentations sometime before the beginning of their talk (preferably as far in advance as possible).

The data and software required for participation in the tutorial sessions can be found at: Workshop2011.

Information on the location of the workshop and where to park is here: Ws2011/Location

The main workshop page is here: http://ncmi.bcm.edu/ncmi/events/workshops/workshops_114

Note that PGH216 is an overflow room for the lectures in case the lecture hall becomes full for the talks. During the tutorial sessions, there will be plenty of seats in PGH232.

Mon Mar 14, 2011

Theme - Single particle reconstruction

8:00 - 8:50 Registration Outside PGH 232
8:50 - 9:10 Opening remarks/Introduction to Workshop PGH232 Dr. Steve Ludtke
9:10 - 10:00 Cryo-EM Essentials PGH232 Dr. Wah Chiu
10:00 - 10:50 Image processing theory PGH232 Dr. Richard Henderson
11:00 - 11:20 Coffee Break
11:20 - 12:30 Tutorial 1 - Getting started with EMAN2, CTF & class-averages PGH232&200 Dr. Steve Ludtke
Tutorial 1 - Slides from presentation
12:30 - 1:30 Lunch
1:30 - 3:00 Tutorial 1, contd - Initial models PGH232&200 Dr. Steve Ludtke
3:00 - 3:20 Coffee Break
3:20 - 5:00 Tutorial 1, contd - First refinement & other issues PGH232&200 Dr. Steve Ludtke
5:00 - 6:00 Research Lecture: Structural studies of TriC/CCT PGH232 Dr. Yao Cong
6:00 - 9:00 Dinner onsite - with participant short talks PGH232

Tue Mar 15, 2011

Theme - Helices & Heterogeneity

9:00 - 10:00 Helical filaments, heterogeneity and polymorphisms PGH232 Dr. Edward Egelman
10:00 - 10:20 Coffee Break
10:20 - 11:20 Identification of conformational states using resampling PGH232 Dr. Pawel Penczek
11:30 - 12:30 Tutorial 2 - Chimera PGH232&200 Tom Goddard
12:30 - 1:30 Lunch
1:30 - 3:00 Tutorial 3 - Evaluating refinements, heterogeneity, etc. PGH232&200 Dr. Steve Ludtke
Tutorial 3 - Presentation slides for tutorial session
3:00 - 3:15 Coffee Break
3:15 - 5:00 Tutorial 4 - Introduction to identification of conformational states in SPARX PGH232&200 Dr. Pawel Penczek
5:15 - 6:15 Research Talk - Multiple states of a chaperonin and a method for analyzing heterogeneous conformations in cryo-EM PGH232 Dr. Junjie (George) Zhang
6:30 - 9:00 Dinner UH Library

Wed, Mar 16, 2011

Theme - Related methods

8:50 - 9:15 Tutorial 5 - Quick morning tutorial session PGH232&200 Stephen Murray
9:15 - 10:00 Single particle tomography PGH232 Dr. Mike Schmid
10:10 - 10:30 Coffee Break
10:30 - 11:00 EMDatabank.org & Data Archival Tutorial/Guide PGH232 Dr. Cathy Lawson
11:00 - 11:45 Tutorial 6 - Segmentation with Segger PGH200&232 Dr. Greg Pintilie
11:45 - 12:45 Lunch
12:45 - 3:00 Tutorial 5 cont'd - Frealign, multirefine, etc. PGH200&232 Stephen Murray & Dr. Steve Ludtke
3:00 - 3:30 Coffee Break
3:30 - 5:00 Tutorial 7 - Single Particle Tomography in EMAN2 Short PPT introPGH200&232 Jesus Galaz & Dr. John Flanagan
5:10 - 6:10 Research Talk - Structural studies of Calcium release channels PGH232 Dr. Irina Serysheva
7:00 - 9:00 Workshop Banquet Bus leaves U of H at 6:30 ! Fung’s Kitchen

Thu, Mar 17, 2011

Theme: Validation & Modeling

9:00 - 10:00 Methods for Validation of Cryo-EM Maps PGH232 Dr. Richard Henderson
10:00 - 10:30 Coffee Break
10:30 - 11:30 Tutorial 8: Real-space Refinement of Atomic Models in DireX PGH200/232 Dr. Gunnar Schroeder
11:30 - 12:15 Tutorial 9: The EMEN2 Electronic Notebook and OODB PGH200/232 Ian Rees
12:15 - 1:00 Lunch
1:00 - 2:30 Tutorial 10: Introduction to Rosetta PGH200/232 Dr. Yao Cong
2:30 - 3:00 Coffee Break
3:00 - 4:30 Tutorial 11: Model Building and Analysis with Gorgon PGH200/232 Dr. Matt Baker
4:30 - 5:30 Workshop wrap-up, final discussion

Workshop Ends