Changing image format from real/imaginary to amplitude/phase format in EMAN2 is a straightforward procedure. To illustrate the process start with a test image and perform an inplace Fourier transform on it as follows: [someone@localhost] e2.py Welcome to EMAN2 Prompt provided by IPython Enter '?' for ipython help In [3]: e = EMData(32,32,32) In [4]: e.process_inplace("testimage.axes") In [5]: e.do_fft_inplace() You now have an EMData object (e) in real/imaginary format. You can verify that the image is in this format by calling the function **is_ri**, for example try: In [6]: e.is_ri() Out[6]: True To convert the image to amplitude/phase format simply do the following: In [7]: e.ri2ap() In [8]: e.is_ri() Out[8]: False In [9]: e.get(2,0,0) Out[9]: 62.0 In [10]: e.get(3,0,0) Out[10]: 3.1415927410125732 Note that it's relatively straightforward to access values from the EMData object (e) using the **get** function call, and that we have verified the EMData object is in amplitude/phase format using the function **is_ri**, as demonstrated above. Finally, you can convert back to to real/imaginary format by doing: In [11]: e.ap2ri() In [12]: e.is_ri() Out[12]: True