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eman2:e2tomo_atpsyn [2026/06/06 03:10] muyuancheneman2:e2tomo_atpsyn [2026/06/11 16:19] (current) muyuanchen
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 Unzip the dataset, and you should have a folder called "tiltseries", with four hdf image stacks in it, inside the project folder. To view the tilt series, run **e2display.py**, locate the file in the browser, and click **Show2D**. Unzip the dataset, and you should have a folder called "tiltseries", with four hdf image stacks in it, inside the project folder. To view the tilt series, run **e2display.py**, locate the file in the browser, and click **Show2D**.
  
-{{:eman2:atpsyn_tilt_series.jpg|tiltseries|width="600"}}+{{:eman2:atpsyn_tilt_series.jpg|tiltseries}}
  
 ===== Initial tomogram reconstruction ===== ===== Initial tomogram reconstruction =====
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 +===== Map particles to tomograms =====
 +
 +As shown in previous tutorials, we can map particles back to the original tomograms to visualize their organization inside the tomogram. 
 +
 +<code>
 +e2spt_mapptclstotomo.py --path=spt_03 --iter=7 --tomo=tomograms/14042022_BrnoKrios_Arctis_grid5_Position_17__bin4.hdf --new
 +</code>
 +
 +Then open the raw tomogram and the mapped back particles (spt_03/ptcls_in_tomo_14042022_BrnoKrios_Arctis_grid5_Position_17_07.hdf) in Chimera. 
 +
 +{{:eman2:atpsyn_map_back.jpg | Particles in tomo | width="600"}}
  
eman2/e2tomo_atpsyn.1780715401.txt.gz · Last modified: by muyuanchen