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NCMI Workshop on Single Particle Reconstruction, Validation and Analysis

March 14 - 17, 2011

This page contains the workshop schedule, and will contain the PDFs of each of the presentations at the workshop. Speakers are asked to upload their presentations sometime before the beginning of their talk (preferably as far in advance as possible).

The data and software required for participation in the tutorial sessions can be found at: Workshop2011.

Mon Mar 14, 2011

Theme - Single particle reconstruction

8:00 - 8:50	Registration	Outside PGH 200
8:50 - 9:10	Opening remarks/Introduction to Workshop	PGH200	Dr. Steve Ludtke
9:10 - 10:00	Introduction to transmission electron microscopy	PGH200	Dr. Wah Chiu
10:00 - 10:50	Image processing theory	PGH200	Dr. Richard Henderson
11:00 - 11:20	Coffee Break
11:20 - 12:30	Tutorial 1 - Getting started with EMAN2, CTF & class-averages	PGH232&200	Dr. Steve Ludtke
12:30 - 1:30	Lunch
1:30 - 3:00	Tutorial 1, contd - Initial models	PGH232&200	Dr. Steve Ludtke
3:00 - 3:20	Coffee Break
3:20 - 5:00	Tutorial 1, contd - First refinement & other issues	PGH232&200	Dr. Steve Ludtke
5:00 - 6:00	Research Lecture: Structural studies of TriC/CCT	PGH200	Dr. Yao Cong
6:00 - 9:00	Dinner onsite - with participant short talks	PGH200

Tue Mar 15, 2011

Theme - Helices & Heterogeneity

9:00 - 10:00	Helical filaments, heterogeneity and polymorphisms	PGH200	Dr. Edward Egelman
10:00 - 10:20	Coffee Break
10:20 - 11:20	Identification of conformational states using resampling	PGH200	Dr. Pawel Penczek
11:30 - 12:30	Tutorial 2 - Chimera	PGH232&200	Tom Goddard
12:30 - 1:30	Lunch
1:30 - 3:00	Tutorial 3 - Evaluating refinements, heterogeneity, etc.	PGH232&200	Dr. Steve Ludtke
3:00 - 3:15	Coffee Break
3:15 - 5:00	Tutorial 4 - Introduction to identification of conformational states in SPARX	PGH232&200	Dr. Pawel Penczek
5:15 - 6:15	Research Talk - Multiple states of a chaperonin and a method for analyzing heterogeneous conformations in cryo-EM	PGH200	Dr. Junjie (George) Zhang
6:30 - 9:00	Dinner	UH Library

Wed, Mar 16, 2011

Theme - Related methods

8:50 - 9:15	Tutorial 5 - Quick morning tutorial session	PGH232&200	Stephen Murray
9:15 - 10:00	Single particle tomography	PGH200	Dr. Mike Schmid
10:10 - 10:30	Coffee Break
10:30 - 11:00	EMDatabank.org & Data Archival	PGH200	Dr. Cathy Lawson
11:00 - 11:45	Tutorial 6 - Segmentation with Segger	PGH200&232	Dr. Greg Pintilie
11:45 - 12:45	Lunch
12:45 - 3:00	Tutorial 5 cont'd - Frealign, multirefine, etc.	PGH200&232	Stephen Murray & Dr. Steve Ludtke
3:00 - 3:30	Coffee Break
3:30 - 5:00	Tutorial 7 - Single Particle Tomography in EMAN2	PGH200&232	Jesus Montoya & Dr. John Flanagan
5:10 - 6:10	Research Talk - Structural studies of Calcium release channels	PGH200&232	Dr. Irina Serysheva
7:00 - 9:00	Workshop Banquet Bus leaves U of H at 6:30 !	Fung’s Kitchen

Thu, Mar 17, 2011

Theme: Validation & Modeling

9:00 - 10:00	Methods for Validation of Cryo-EM Maps	PGH200	Dr. Richard Henderson
10:00 - 10:30	Coffee Break
10:30 - 11:30	Real-space Refinement of Atomic Models in DireX	PGH200/232	Dr. Gunnar Schroeder
11:30 - 12:15	The EMEN2 Electronic Notebook and OODB	PGH200/232	Ian Rees
12:15 - 1:00	Lunch
1:00 - 2:30	Introduction to Rosetta	PGH200/232	Dr. Yao Cong
2:30 - 3:00	Coffee Break
3:00 - 4:30	Model Building and Analysis with Gorgon	PGH200/232	Dr. Matt Baker
4:30 - 5:30	Workshop wrap-up, final discussion

Workshop Ends

Note: Wah will host a dinner for any speakers who will be around Thurs evening

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||12:45 - 3:00 ||Tutorial 5 cont'd - Frealign, multirefine, etc. ||PGH200&232 ||Dr. Steve Ludtke & Stephen Murray ||
||3:00 - 3:30 ||Coffee Break ||
+||11:45 - 12:45 ||Lunch || || ||
||12:45 - 3:00 ||Tutorial 5 cont'd - Frealign, multirefine, etc. ||PGH200&232 || Stephen Murray & Dr. Steve Ludtke ||
||3:00 - 3:30 ||Coffee Break || || ||
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-||7:00 - 9:00 ||Workshop Banquet '''Bus leaves U of H at 6:30 !''' ||Fung’s Kitchen||
+||7:00 - 9:00 ||Workshop Banquet '''Bus leaves U of H at 6:30 !''' ||[[http://www.fungskitchen.com|Fung’s Kitchen]]|| ||
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