|
⇤ ← Revision 1 as of 2007-07-11 17:37:30
Size: 617
Comment:
|
Size: 840
Comment:
|
| Deletions are marked like this. | Additions are marked like this. |
| Line 1: | Line 1: |
| This is largely answered in the question above relating to resolution of reconstructions. If you are not using optimal options with the refine command, you may, indeed, not get optimal results. The basic options provided by the EMAN tutorial do not give the entire picture. For example, if you continue to use classiter=8 for your high resolution work, you will never get the highest possible resolution. Similarly, if you don't use the refine option to the refine command, you will end up slightly blurring your model in most cases. The bottom line is, to get optimal results, you must specify the optimal options. | ''' ''''''Question:''' What sort of problems will EMAN have with initial model bias ? What if I can't get my structure to converge to something sensible in EMAN, but I can in another program ? ''' ''' '''Answer: '''This is largely answered in the question above relating to resolution of reconstructions. If you are not using optimal options with the refine command, you may, indeed, not get optimal results. The basic options provided by the EMAN tutorial do not give the entire picture. For example, if you continue to use classiter=8 for your high resolution work, you will never get the highest possible resolution. Similarly, if you don't use the refine option to the refine command, you will end up slightly blurring your model in most cases. The bottom line is, to get optimal results, you must specify the optimal options. |
Question: What sort of problems will EMAN have with initial model bias ? What if I can't get my structure to converge to something sensible in EMAN, but I can in another program ?
Answer: This is largely answered in the question above relating to resolution of reconstructions. If you are not using optimal options with the refine command, you may, indeed, not get optimal results. The basic options provided by the EMAN tutorial do not give the entire picture. For example, if you continue to use classiter=8 for your high resolution work, you will never get the highest possible resolution. Similarly, if you don't use the refine option to the refine command, you will end up slightly blurring your model in most cases. The bottom line is, to get optimal results, you must specify the optimal options.