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| = EMAN2 = | = EMAN2.2 = Most of the pages are editable by any user that has registered an account on the server. To prevent spam, you need to email sludtke@bcm.edu to get an account on the system if you wish to contribute changes. If you just wish to browse, you don't need an account. |
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| EMAN2 is the successor to [[EMAN1|EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1). | EMAN2 is the successor to [[EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1). |
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| Please also note that this is NOT the (related) [[EMEN2]] electronic notebook, but is EMAN2, a scientific image processing suite. | Please also note that this is '''not''' the (related) [[EMEN2]] electronic notebook, but is EMAN2, a scientific image processing suite. |
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| '''Note for EMAN1 users - ''' If you are still using EMAN1 due to some missing functionality in EMAN2, please email sludtke@bcm.edu or post a message on the Google group telling what you are still doing in EMAN1. We will either explain how to do it in EMAN2, or add it to the list of projects. Please also note that there is an EMAN1->EMAN2 transition guide available below. Thanks ! | '''''The official 2.2 release is still a work in progress, but should be complete by the end of Feb 2017''''' |
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| = Download EMAN2 = | == Please Cite == EMAN is free software, supported by NIH Grants. To keep our grant funding it is critical that you cite EMAN2 when you use it in a publication in any significant way. * Primary EMAN2 paper: * G. Tang, L. Peng, P.R. Baldwin, D.S. Mann, W. Jiang, I. Rees & S.J. Ludtke. (2007) EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol. 157, 38-46. PMID: 16859925 * EMAN2 high resolution refinement methods: * J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke. (2016) High Resolution Single Particle Refinement in EMAN2.1. Methods. 100, 25-34. PMID: 26931650 * Methods for analysis of conformational and compositional variability: * Ludtke, S. J. "Single-Particle Refinement and Variability Analysis in EMAN2.1." in Methods Enzymol 579159-189 (Elsevier, United States, 2016). * Methods for subtomogram averaging: * J.G. Galaz-Montoya, C.W. Hecksel, P.R. Baldwin, E. Wang, S.C. Weaver, M.F. Schmid, S.J. Ludtke & W. Chiu. (2016) Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography. J Struct Biol. 194, 383-394. PMC4846534. |
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== Download EMAN2 == * [[EMAN2/Eman22Release|EMAN2.2 Release Notes]] |
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| * [[http://ncmi.bcm.edu/ncmi/software/counter_222/software_86|Nightly builds - direct link]] (<-- We normally suggest using this version, which is updated every night) * [[http://ncmi.bcm.edu/ncmi/software/counter_222/software_120|Last stable version - direct link]] |
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| = Install EMAN2 = | == Install EMAN2 == |
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| * Please check this [[EMAN2/ChangeLog|CHANGELOG]] after upgrading to a new version * '''''Please read this [[EMAN2/DatabaseWarning|Important Warning]]''''' |
* [[EMAN2/Remote|Strategies for using the GUI remotely (clusters or remote workstations)]] |
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| = Documentation = | == Documentation == * Live Tutorials * Archived video tutorials: [[https://www.youtube.com/c/SteveLudtke]] |
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| * [[EMAN2/Eman1Transition|EMAN1 -> EMAN2 Transition Guide]] * [[EMAN2/Tutorials|EMAN2 Tutorials (START HERE!)]] * [[EMAN2/VideoTutorials|EMAN2 Video Tutorials]] * [[EMAN2/Concepts|File Formats, Image Processing Functions, Mathematical Conventions]] |
* [[EMAN2/Tutorials|Tutorials (START HERE!)]] * [[EMAN2/DirectoryStructure|Folders and files in an EMAN2 Project]] * [[EMAN2/Concepts|File Formats, Symmetry, Box Size, ...]] |
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| * [[EMAN2/Eman1Transition|EMAN1 -> EMAN2 Transition Guide]] | |
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| * [[EMAN2/Obsolete|Obsolete Pages]] | |
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| * [[http://blake.grid.bcm.edu/eman2/doxygen_html/classEMAN_1_1EMData.html|Direct link to docs for EMData (image) class]] * [[http://blake.grid.bcm.edu/eman2/doxygen_html/classEMAN_1_1Transform.html|Direct link to docs for Transform (orientation/Euler angle) class]] * [[EMAN2/GitTutorials|Transitioning from CVS to Git (under construction)]] |
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| = About EMAN2 = EMAN2 is the successor to [[EMAN1|EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display. |
== About EMAN2 == EMAN2 is the successor to [[EMAN1]]. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display. |
EMAN2.2
Most of the pages are editable by any user that has registered an account on the server. To prevent spam, you need to email sludtke@bcm.edu to get an account on the system if you wish to contribute changes. If you just wish to browse, you don't need an account.
EMAN2 is the successor to EMAN1. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1).
Please also note that this is not the (related) EMEN2 electronic notebook, but is EMAN2, a scientific image processing suite.
The official 2.2 release is still a work in progress, but should be complete by the end of Feb 2017
Please Cite
EMAN is free software, supported by NIH Grants. To keep our grant funding it is critical that you cite EMAN2 when you use it in a publication in any significant way.
- Primary EMAN2 paper:
G. Tang, L. Peng, P.R. Baldwin, D.S. Mann, W. Jiang, I. Rees & S.J. Ludtke. (2007) EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol. 157, 38-46. PMID: 16859925
- EMAN2 high resolution refinement methods:
J.M. Bell, M. Chen, P.R. Baldwin & S.J. Ludtke. (2016) High Resolution Single Particle Refinement in EMAN2.1. Methods. 100, 25-34. PMID: 26931650
- Methods for analysis of conformational and compositional variability:
- Ludtke, S. J. "Single-Particle Refinement and Variability Analysis in EMAN2.1." in Methods Enzymol 579159-189 (Elsevier, United States, 2016).
- Methods for subtomogram averaging:
J.G. Galaz-Montoya, C.W. Hecksel, P.R. Baldwin, E. Wang, S.C. Weaver, M.F. Schmid, S.J. Ludtke & W. Chiu. (2016) Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography. J Struct Biol. 194, 383-394. PMC4846534.
Download EMAN2
Install EMAN2
Documentation
- Live Tutorials
Archived video tutorials: https://www.youtube.com/c/SteveLudtke
- User Documentation
Advanced Users & Programmers
FAQ - Please ask your questions in the Google Group, answers to common questions will be posted here as well as in the Group
- Mailing list for EMAN2 discussions:
eman2@googlegroups.com (must join the group before you can post)
http://groups.google.com/group/eman2-developers (This is the group for discussions among developers, likely less interesting for users)
About EMAN2
EMAN2 is the successor to EMAN1. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. Image processing in a suite like EMAN differs from consumer image processing packages like Photoshop in that pixels in images are represented as floating-point numbers rather than small (8-16 bit) integers. In addition, image compression is avoided entirely, and there is a focus on quantitative analysis rather than qualitative image display.
EMAN2 is now a fully usable reconstruction package, including parallelism support. However, in case you aren't ready to abandon EMAN1 yet, EMAN1 and EMAN2 can be installed in the same user account with no conflicts. All EMAN2 programs, including GUI programs, are written in the easy-to-learn Python scripting language. This permits knowledgeable end-users to customize any of the code with unprecedented ease. If you aren't an advanced user, you can still make use of the integrated GUI and all of EMAN2's command-line programs. Any programs in EMAN2 with an EMAN1 equivalent are likely substantially improved over their EMAN1 equivalents. For example e2pdb2mrc.py is ~10x faster than the EMAN1 pdb2mrc. 3-D refinements are typically 5-25x faster than in EMAN1 (with the correct options).
