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| are planned but the tutorials have not yet been completed. If you have a request for a particular tutorial | are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial |
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| ==== Single particle analysis (SPA) - 2D particles==== | === Video tutorials and discussions === * [[EMAN2/VideoTutorials|EMAN2 Video Tutorials]] |
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| * [[EMAN2/Eman1Transition|EMAN1 to EMAN2 Transition Guide]] * The old SPR tutorial - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]]) * [[Ws2011/Eman2|The NEW tutorial from the 2011 EMAN2 workshop, compatible with EMAN 2.01 (Data on same page)]] * [[attachment:rct_tutorial.pdf|Tutorial on using EMAN2 for Random Conical Tilt reconstructions]] |
=== Written Tutorials === |
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| ==== Single particle tomography (SPT) - 3D particles==== * NEW single particle tomography USERS' GUIDE. [Partially used for the SPT tutorial at the 2011 EMAN2 workshop]. [[attachment:e2spt_users_guide.pdf| e2spt_users_guide.pdf|&do=get]] * UPDATED on April 18, 2011. '''It SHOULD be a valid PDF'''. If not, please report it, and try to open in with OpenOffice or Word. |
==== Single particle analysis (SPA) - 2D particles ==== * 2017 February Tutorial for EMAN2.2 * [[attachment:EMAN2-2Tutorial.pdf||&do=get]] * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]] * 2016 Summer Tutorial for EMAN2.12 June 2016 snapshots * [[attachment:EMAN2-13_pre_Tutorial.pdf||&do=get]] * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]] * [[attachment:EMAN2-13_pre_Heterogeneity.pdf||&do=get]] * see also: http://www.sciencedirect.com/science/article/pii/S0076687916300362 * 2015 Fall Tutorial for EMAN2.12 * [[attachment:EMAN2-12_Tutorial.pdf||&do=get]] * Required data - [[attachment:Bgal.zip||&do=get]] * [[attachment:EMAN2-12_Tutorial2_heterogeneity.pdf||&do=get]] * Required data - [[attachment:ribosome.zip||&do=get]] * [[Ws2015|2015 Workshop Tutorials and Demo data]] - This includes updated tutorials on both high resolution single particle analysis and 2D/3D heterogeneity analysis along with sample data, freshly updated for the EMAN2.12 release. The workshop also included a number of other related topics also available on that page. |
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| * [[EMAN2/Programs/convnet_pickparticle|tutorial for the Neural network particle picker]] (requires CUDA for good performance) * [[EMAN2/Eman1Transition|EMAN1 to EMAN2 Transition Guide]] - Also with useful quickstart guides for users of other software * [[OxfordWs2012|Tilt Validation tutorial]] * [[RctTutorial|Random Conical Tilt Tutorial]] |
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| ==== 2D assessment of heterogeneity ==== * [[attachment:population_dynamics_2009_11.pdf|Full tutorial on 2-D analysis for assessment of structural heterogeneity and population dynamics]] |
==== Single particle tomography (SPT) - 3D particles, also called subtomogram averaging ==== BOTH the tutorial in .pdf format and the data needed for the tutorial in a .zip file are provided in the page linked below: * [[SPT/Spt|Single particle tomography]] ==== Automated Cellular Tomogram Annotation using Neural Networks ==== * [[EMAN2/Programs/tomoseg|Tomogram Annotation]] ==== Using the Built-In Modules from Other Software ==== * [[EMAN2/Programs/e2refinetorelion2d/e2refinetorelion2dtutorial|2D Class Averaging with Relion]] * [[EMAN2/Programs/e2refinetorelion3d/e2refinetorelion3dtutorial|3D Refinement with Relion]] * [[EMAN2/Programs/e2refinetofrealign/e2refinetofrealigntutorial|3D Refinement with FreAlign]] === Out of date tutorials (provided for data access, etc.) === * [[Ws2014|Tutorial from the 2014 Workshops in Shanghai, Brisbane and online]] - Based on EMAN2.1 beta4. Full single particle reconstruction tutorial * Single particle reconstruction tutorial from 2008 Workshop ( - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]]) * [[Ws2011/Eman2|Tutorial from the 2011 EMAN2 workshop (Data on same page)]] - Based on EMAN2.01. Full single particle reconstruction tutorial * [[Ws2012/EMAN2|Tutorial from the 2012 Workshop in Singapore (Data on same page)]] - Based on EMAN2.06. Full single particle reconstruction tutorial * [[Ws2013/EMAN2|Tutorial from the 2013 Workshop in Beijing (Data on same page)]] - Based on EMAN2.1alpha. Full single particle reconstruction tutorial |
EMAN2 TUTORIALS
Beginners (introductory tutorials)
Please note that we are continuously developing and updating the tutorials. Items that are greyed out are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial please feel free to email sludtke@bcm.edu.
Video tutorials and discussions
Written Tutorials
Single particle analysis (SPA) - 2D particles
- 2017 February Tutorial for EMAN2.2
- 2016 Summer Tutorial for EMAN2.12 June 2016 snapshots
- 2015 Fall Tutorial for EMAN2.12
Required data - Bgal.zip
Required data - ribosome.zip
2015 Workshop Tutorials and Demo data - This includes updated tutorials on both high resolution single particle analysis and 2D/3D heterogeneity analysis along with sample data, freshly updated for the EMAN2.12 release. The workshop also included a number of other related topics also available on that page.
tutorial for the Neural network particle picker (requires CUDA for good performance)
EMAN1 to EMAN2 Transition Guide - Also with useful quickstart guides for users of other software
Single particle tomography (SPT) - 3D particles, also called subtomogram averaging
BOTH the tutorial in .pdf format and the data needed for the tutorial in a .zip file are provided in the page linked below:
Automated Cellular Tomogram Annotation using Neural Networks
Using the Built-In Modules from Other Software
Out of date tutorials (provided for data access, etc.)
Tutorial from the 2014 Workshops in Shanghai, Brisbane and online - Based on EMAN2.1 beta4. Full single particle reconstruction tutorial
Single particle reconstruction tutorial from 2008 Workshop ( - Full tutorial on single particle reconstruction using the workflow (uses Workshop demo data DVD)
Tutorial from the 2011 EMAN2 workshop (Data on same page) - Based on EMAN2.01. Full single particle reconstruction tutorial
Tutorial from the 2012 Workshop in Singapore (Data on same page) - Based on EMAN2.06. Full single particle reconstruction tutorial
Tutorial from the 2013 Workshop in Beijing (Data on same page) - Based on EMAN2.1alpha. Full single particle reconstruction tutorial
Advanced (EMAN2 through the ''e2.py'' Python prompt)
* Generate and display an EMAN2 test image
* Rotate, translate, scale or mirror an image
* Add and multiply images, add and multiply by constants
* Convert a real/imaginary image into an amplitude/phase image and vice versa
* Iterate through the pixels of an image
* Make a projection of a 3D model, project through the asymmetric unit
