What follows is a very simply demonstration of using the Transform object to transform EMData (image) objects. A very thorough explanation using the Transform is presented in Using the EMAN2 Transform class and you are encouraged to at least be aware of that page's existence.
[someone@localhost]$ e2.py
Welcome to EMAN2
Prompt provided by IPython
Enter '?' for ipython help
In [3]: e = EMData()
In [4]: e.set_size(32,32,32)
In [5]: e.process_inplace('testimage.axes')
In [6]: t = Transform({"type":"eman","az":45,"alt":90})
In [7]: t.set_trans(1,-2,5)
In [8]: t.set_scale(1)
In [9]: e.transform(t)
In [9]: display(e)