attachment:ctf.png

e2ctf

To get familiar with how e2ctf.py works, try separating the way you using e2ctf.py into three stages:

Auto fitting

e2ctf.py  1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit

Fine tuning

(save any changes you make with the Save Parms button)

e2ctf.py  1.img --gui

Write output

e2ctf.py 1.img --phaseflip --wiener

The output will be in the particles directory. Also, you can supply more than one image as input to e2ctf.py.

You can combine more than command would have worked if you had used a command like this:

e2ctf.py 1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui