Differences between revisions 1 and 21 (spanning 20 versions)

e2ctf

Contents

e2ctf

CTF processing in 3 stages

To get familiar with how e2ctf.py works, try separating the way you using e2ctf.py into three stages:

Auto fitting

e2ctf.py 1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit

This runs auto CTF parameter determination, storing the result in a local database.

Fine tuning

e2ctf.py 1.img --gui

Now that you have determined the parameters with the first command, have a look at the results in the interface. Tweak if necessary. Save any changes you make with the Save Parms button

Write output

e2ctf.py 1.img --phaseflip --wiener

Finally generate output. The output will be in the particles directory.

Processing multiple images simultaneously

e2ctf takes multiple images as input, so you can use commands like this:

e2ctf.py *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit
e2ctf.py *.img --gui
e2ctf.py *.img --phaseflip --wiener

Combining stages into a single command

You can combine more than one of the e2ctf processing stages into a single command. For instance you can run automated fitting and open the GUI with a single command like this:

e2ctf.py 1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui
e2ctf.py *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui

-  ⇤ ← Revision 1 as of 2009-03-02 16:53:04 → 
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+   ← Revision 21 as of 2009-03-02 21:29:55 → ⇥
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-e2ctf
+= e2ctf =

||<<TableOfContents>>||{{attachment:ctf.png}}||

== CTF processing in 3 stages ==

To get familiar with how  e2ctf.py works, try separating the way you using e2ctf.py into three stages:

=== Auto fitting ===

{{{
e2ctf.py 1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit
}}}

This runs auto CTF parameter determination, storing the result in a local database.

=== Fine tuning ===


{{{
e2ctf.py 1.img --gui
}}}

Now that you have determined the parameters with the first command, have a look at the results in the interface. Tweak if necessary. Save any changes you make with the Save Parms button

=== Write output ===

{{{
e2ctf.py 1.img --phaseflip --wiener
}}}

Finally generate output. The output will be in the particles directory.

== Processing multiple images simultaneously ==

e2ctf takes multiple images as input, so you can use commands like this:

{{{
e2ctf.py *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit
e2ctf.py *.img --gui
e2ctf.py *.img --phaseflip --wiener
}}}

== Combining stages into a single command ==

You can combine more than one of the e2ctf processing stages into a single command. For instance you can run automated fitting and open the GUI with a single command like this:

{{{
e2ctf.py 1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui
e2ctf.py *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui
}}}