Diff for "EMAN1/FAQ/FSC_FSC"

Differences between revisions 1 and 3 (spanning 2 versions)

Question: I am having problems reading mrc maps in EMAN. I have a ccp4 map which I convert to mrc using em2em. The map looks fine in chimera. when I am trying to use this file by lauching ssehunter3.py, the reading of the map is starting OK but then I got this error message:

Map Information

mean: -0.003090 sigma: 0.078643 threshold: 0.100000 dimensions:22,22,22

Warning: Incomplete data read 14/484 blocks

-12.0 12.0 Segmentation fault (core dumped)

I have also tried to convert with situs but without success. Is there any way I can get a ccp4 map converted to an mrc that is readable by eman?

-  ⇤ ← Revision 1 as of 2007-06-07 14:31:53 → 
  Size: 835
  Editor: SteveLudtke
  Comment:
+   ← Revision 3 as of 2007-06-19 09:56:13 → ⇥
  Size: 646
  Editor: lcr2pc1
  Comment:
-Deletions are marked like this.
+Additions are marked like this.
 Line 1:
-I am having an issue with the eotest and FSC curves.  When the
particles are low pass filtered, the FSC curve produced by eotest starts to
come up sometimes quite significantly, to around 0.5, at about the
resolution frequency that the particles were filtered at.  Also, the
same thing happens when the models are CTF corrected.  I have tested
the effect of specifying different CTF correction cutoffs on the FSC
curve at 20, 30, and 40A.  Each time the curve produced by eotest
starts to increase just before the cutoff.  The same behavior is
observed with the particles low passed using different low pass
filters.
+'''Question:''' 
I am having problems reading mrc maps in EMAN.
I have a ccp4 map which I convert to mrc using em2em.
The map looks fine in chimera.
when I am trying to use this file by lauching ssehunter3.py, the reading of the map is starting OK but then I got this error message:
-Line 12:
+Line 7:
-My particles are negatively stained so I need to do a low pass filter
for the reconstruction and the CTF correction is to a low resolution.
Do you have any suggestions on how to eliminate this behavior?
+Map Information
   mean:      -0.003090
   sigma:     0.078643
   threshold: 0.100000
   dimensions:22,22,22
Warning: Incomplete data read 14/484 blocks

-12.0 12.0
Segmentation fault (core dumped)

I have also tried to convert with situs but without success.
Is there any way I can get a ccp4 map converted to an mrc that is readable by eman?